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課程名稱 |
蛋白質結構與動力學
Protein Structure and Dynamics |
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開課學期 |
112-1 |
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授課對象 |
理學院 化學所化學生物學組 |
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授課教師 |
馬左仲 |
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課號 |
Chem5127 |
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課程識別碼 |
223EU1170 |
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班次 |
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學分 |
3.0 |
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全/半年 |
半年 |
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必/選修 |
選修 |
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上課時間 |
星期三7,8,9(14:20~17:20) |
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上課地點 |
化121 |
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備註 |
本課程以英語授課。
限學士班三年級以上 且 限本系所學生(含輔系、雙修生)
總人數上限：30人 |
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課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
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課程大綱
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為確保您我的權利,請尊重智慧財產權及不得非法影印
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課程概述 |
In this course, students will get an introduction to the principles of 3D structures of proteins, which underlie all protein function, as well as the techniques used to obtain and analyze protein structures. There will be a strong emphasis on protein dynamics as well, i.e. how protein structural flexibility permits enzymatic/receptor/structural activity.
Finally, there will be a strong hands-on experience for all students, with in-silico analysis of protein structure, motion and activity. |
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課程目標 |
Students who successfully pass this course will have an overview of the field of protein biophysics and structure, with a strong emphasis on practical analysis and structural evaluation. |
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課程要求 |
Students shouldbring a virtualbox-compatible computing device (windows, mac, and linux work, not sure about iOS or Android), with virtualbox installed (https://www.virtualbox.org/wiki/Downloads). I will provide a virtual machine with all required software for students to work during the practical part of the course. |
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預期每週課後學習時數 |
For every hour of class, I expect students to have to review the materials for another hour. |
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Office Hours |
每週四 15:00~17:00 備註： I am open to appointments for students with busy schedules |
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指定閱讀 |
No overall designated reading, but for every class a list of references will be provided. |
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參考書目 |
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評量方式
(僅供參考) |
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週次 |
日期 |
單元主題 |
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Week 1 |
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Introduction, installation of virtual machines, playing with structures |
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Week 2 |
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The hierarchy of protein structure I: Overview and the nature of amino-acids |
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Week 3 |
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The hierarchy of protein structure II: Secondary and Tertiary structure |
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Week 4 |
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Protein folding: Anfinsen boxes and folding dynamics |
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Week 5 |
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Water |
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Week 6 |
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The hierarchy of protein structure III: Quaternary structure and cooperativity |
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Week 7 |
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Virtual visit to an XFEL facility |
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Week 8 |
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Midterm |
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Week 9 |
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In silico protein structure: Molecular Dynamics & Alphafold (guest instructor) |
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Week 10 |
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Introduction to protein structural techniques: CryoEM and NMR |
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Week 11 |
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X-ray crystallography I: Theory of diffraction |
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Week 12 |
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X-ray crystallography II: Data Acquisition and Structure Refinement |
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Week 13 |
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Introduction to time-resolved crystallography: a new era in protein dynamics |
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Week 14 |
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Time-resolved analysis of protein structure: 3D movies of proteins at work |
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Week 15 |
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Time-resolved analysis of protein structure: the intersection between quantum chemisitry and protein folding |
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Week 16 |
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Final |
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